Measuring Dissolved Hydrogen
H2Blue is a reagent designed to detect the presence of dissolved hydrogen gas in water by performing what chemists call a “titration”. In a titration, a substance of known concentration (theH2Blue reagent) is added to a measured volume of water containing an unknown level of dissolved hydrogen gas (H2) . In the presence of dissolved hydrogen gas, the H2Blue molecules bond with the dissolved H2 molecules, causing the blue molecules to turn “clear”. As additional drops are added, the available dissolved hydrogen molecules are “consumed” until there are no more dissolved H2 molecules in the water. This is called the “titration endpoint”. When the endpoint has been reached, additional drops will no longer turn clear and the solution will remain blue. By counting the number of drops of H2Blue required to reach the titration endpoint in a 6 mL sample of water, and then multiplying the number of drops by 0.1, the level of dissolved H2 gas in parts per million (ppm) can be determined. For example, 6 drops x 0.1 = 0.6 ppm of dissolved H2. (see PPM Page for an explanation of parts per million)
Demonstrating the Presence of Antioxidants
H2Blue is a reagent designed to detect the presence of dissolved hydrogen gas, the therapeutic antioxidant in ionized water (as well as water from other devices which produce hydrogen-rich water). In the presence of dissolved hydrogen gas (H2), the molecules contained inH2Blue bond with the dissolved H2 gas, the H2Blue molecules to turn “clear”. When a drop ofH2Blue is added to water containing an adequate level of dissolved H2 (> 0.1ppm), the drop turns clear almost immediately. So-called “antioxidant” bottled waters will not cause H2Blue to turn clear if they do not contain dissolved hydrogen gas. (Clear plastic bottles cannot hold hydrogen gas for longer than a few days).
Insuring Peak Performance of H2 Water Devices
When hydrogen water devices are new, they may produce good levels of dissolved hydrogen gas (H2). But, as calcium contained in the source water begins to accumulate on the electrodes and hoses, the dissolved hydrogen levels can begin to drop, sometimes in as little as a few weeks. By using H2Blue to measure dissolved H2 levels, you can be sure that your device is performing at peak levels. H2Blue can also be used to measure the dissolved H2 levels in water produced by other devices including hydrogen tablets, magnesium sticks, and hydrogen-water filters.
The H2Blue bottle contains 10 mL of reagent and will dispense approximately 450 drops (a typical test for dissolved hydrogen gas will take between 4 and 10 drops). Since bottle capacity is approximately 13 mL, it will not be completely full when received (bottles are filled to 10 mL +/- 0.1 mL using precision dispensing equipment). H2Blue comes in an LDPE plastic bottle with a child-resistant cap. The H2Blue bottle comes stored in a plastic graduated beaker with a screw cap. The beaker is used to measure the precise volume of hydrogen water (6 mL) required for accurate testing of dissolved H2 levels. H2Blue should be stored in a cool, dry place, and should be used within 12 months of purchase.
Helpful Hints for Measuring
*When measuring H2 water from water ionizers or hydrogen infusion machines, be sure to run the device for at least 20 seconds before taking the 6ml sample. This will insure that you are sampling fresh H2 water, not water which has been sitting in the machine.
*The H2Blue spout is designed to dispense a precise droplet size for testing dissolved H2 levels. When dispensing each drop, turn the bottle completely upside down, squeeze the bottle until one drop exits the spout, then release the pressure (which creates a small vacuum). Repeat this for each ensuing drop. Dispensing H2Blue in this way will help to prevent excess reagent from running down the spout.
*After completing each measurement, be sure to completely rinse the beaker to remove all H2Blue residue. If H2Blue residue is permitted to accumulate on the inside of the graduated beaker, this may result in inaccurate H2 readings.
*Because dissolved hydrogen gas starts to escape immediately after the 6mL sample is taken, try to perform measurements as quickly as possible. Multiple drops of H2Blue can be added to save time, but do not add too many, which may cause you to pass the titration endpoint. Best results will be obtained when a measurement takes 3 minutes or less.
*Fill the beaker directly from the device without using any type of intermediate container such as a drinking glass, and hold the beaker at a slight angle while filling to prevent any splashing which may inadvertently force dissolved hydrogen gas out of the water.
*When doing measurements, avoid shaking the beaker vigorously, which can significantly reduce the dissolved H2. It is better to “gently swirl” or use a small straw (such as a coffee stirrer or toothpick) to slowly stir the drops into the sample water.
*When approaching the titration endpoint, be sure to count the last drop which causes the 6mL test sample to remain blue.
TheH2Blue reagent has been tested for performance accuracy in the pH range of 4 to 9.5. Although H2Blue will still detect dissolved H2 outside of this range, results may be skewed or reaction times altered.
What If My H2 Readings are Lower than Expected?
Unless your water contains redox agents which may interfere with the reading (see above), H2Blue will react with dissolved hydrogen gas and turn clear, provided that the dissolved concentration is at least equal to the lower detection level of 0.1PPM. If your alkaline ionizer device is not producing detectable dissolved H2 levels, check for the following:
1) Review tips for getting best results (see “Helpful Hints For Measuring” above)
2) Limescale (from hard water) may have accumulated on the electrodes or plumbing- follow manufacturer’s instructions for cleaning
3) Source water mineral concentrations may be too low for adequate H2 production-
add a mineral pre-filter cartridge to boost source-water conductivity
4) The device may be malfunctioning, preventing the production of H2 gas-contact manufacturer for troubleshooting procedures Staining
H2Blue contains methylene blue, a compound used as a biological tissue dye. Therefore, H2Blue can stain any surface it touches (possibly permanently, depending on porosity). It is recommended that skin, counter-tops, floors, clothing and other surrounding surfaces be protected from inadvertent splashing during use. The user should wear safety glasses to protect eyes from splashing. Use of a mild abrasive cleanser will usually remove stains from most surfaces. Use soap & water to remove from skin.
A Note about the Formulation
H2Blue contains a common dye, methylene blue, a compound used in biological applications. But, a solution of methylene blue by itself WILL NOT react with dissolved hydrogen. The reaction requires the presence of a catalyst to occur. The catalyst is, by weight, the most expensive component in H2Blue.
H2Blue is warranted to perform as described here for one year* from date of purchase. In the event that you are dissatisfied with the product, we will replace it or issue a refund at our discretion. Shipping charges are not covered by warranty and are not refundable. Please contact us using our Contact Page for more information.